Journal: bioRxiv
Article Title: Laminar profile of task-related plasticity in ferret primary auditory cortex
doi: 10.1101/354910
Figure Lengend Snippet: Awake-behaving experimental paradigm and electrode depth registration. a. Head-fixed preparation. Ferrets were implanted with a metal post that was attached to the skull and held fixed during awake-behaving neurophysiological experiments. The ferret performed the task while we recorded from primary auditory cortex (A1) using a 24 channel linear electrode array (Plexon U-probe). b. Pure-tone detection task. Two ferrets were trained to do a conditioned avoidance Go/No-Go pure-tone detection task. In each trial of the task, the animal heard a sequence of reference noises followed by a pure-tone target. Reference noises were “Go” signals, during which the animal was free to lick a waterspout. Upon detecting the target (the “No-Go” signal), the animal stopped licking the water spout to avoid a mild shock. The target frequency was different for each experiment. c. Electrode depth-registration. The left panel shows an example of how the layer 2/3 (L2/3) vs. layers 4-6 (L4-6) border (dashed line) was computed for a single penetration of the 24 channel linear electrode array in A1. Local field potential (LFP) responses to 100 ms tones were used to find a common marker of depth across penetrations (i.e., for depth registration). Registration began by first identifying the electrode with the shortest LFP response latency (Eτ, white square), then finding the LFP waveform correlation coefficients (ρ) between Eτ and all other electrodes in the same penetration. The border between the first neighboring electrode pair with positive and negative correlation coefficients defined the L2/3 vs. L4-6 border , , , – . Laminar profiles were averaged across penetrations after first aligning to the border. The right panel shows the average depth-registered LFP laminar profile in response to 100 ms tones.
Article Snippet: We studied the laminar profile of rapid task-related plasticity by recording from a 24 channel linear electrode array (Plexon U-probe) inserted through the dura, orthogonal to the surface of A1, in two ferrets that were performing an auditory detection task ( ).
Techniques: Sequencing, Marker
